A list of aircraft operators that have that have suffered an accident, serious incident or hijacking. The list may also contain defunct airlines or airlines that in the meantime have changed their name or have merged.

Airlines:
Aeromarket Express (renamed ARM in 1992)
Air Europa
Air Europa Express (Aeronova until 2016)
Air Nostrum
Air Truck / DHL
Airnor - Aeronaves del Noroeste
AlbaStar
Alpa Servicios Aéreos
ARM (named Aeromarket Express before 1992)
Aviaco
Binter Canarias
Binter Méditerraneo (integrated into Air Nostrum)
Bravo Airlines
Canarias Airlines
Canaryfly
Compañía de Líneas Aéreas Subvencionadas S.A. - CLAS
Drenair
Euro Continental Air
Evelop Airlines
Flightline
Futura International Airways
GIR Jet
Gowair
Helitt Líneas Aéreas
HISMA - Sociedad Hispano-Marroqui de Transportes (Secretly funded and equipped by German Luftwaffe)
Hola Airlines (Baleares Link Express, dba Hola Airlines)
Iberia
Iberia Express
Ibertrans Aérea
Ibiza Flights
LAPE - Líneas Aéreas Postales Españolas
LTE International Airways
MAC Aviation
Mayoral Executive Jet
Medit Air Vuelos Mediterraneo
NAYSA Aerotaxis
Orbest Orizonia Airlines (formerly Iberworld)
Paukn Air
Privilege Style
Regional Lineas Aéreas (Subsidiary Gestair and Regional Airlines)
Sky Service Aviation
Spanair
Spantax
Swiftair
Tadair
TAE/Trabajos Aéreos y Enlaces
TAG Aviation España
TASSA - Trabajos Aéreos del Sahara, SA
Viva Air
Volotea
Vueling
WAMOS Air
World2fly
Zorex Air Transport

Corporations:
Aluminios Cortizo

Military:
Aviación Nacional (military air unit supporting General Franco in the Spanish Civil War)
Ejército del Aire
Fuerzas Aéreas República Española - FARE

Organisations (non-profit):
Auxiliar de Actividades Aérea

Special operations (survey, crop spraying etc.):
Aerovisión